Abstract
Chromosomal data of Hyphessobrycon anisitsi are presented for the first time, comprising 2 populations from adjacent hydrographical basins (Upper Parana basin and Paraiba do Sul basin), separated by Mantiqueira Hills (water divisor). The same diploid number and karyotype formula were observed in both populations. However, after silver nitrate staining (Ag-NORs) and fluorescent in situ hybridization with 18S rDNA and 5S rDNA probes, it was possible to identify each population by the location of ribosomal sites. Some aspects related to geographical isolates and chromosomal differentiation are approached.
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