Abstract
In this study we investigated the influence of low dosage X - ray irradiation on the incidence of chromosomal damage and changes in mitotic index (MI) in cultured peripheral lymphocytes of the Bosnian and Herzegovinian mountain horse following in vitro irradiation. X-ray irradiation induced a dose-dependent decrease in MI but only the dose of 0.5 Gy induced a significant decrease (p<0.05) in comparison with the control and other dose groups. The analysis of chromosomal damage revealed a clear dose-dependent increase in the incidence of chromosomal damage per metaphase. Significant differences (p<0.05) were detected by analysis of variance and the LSD test confirmed significant differences between cells that received 0.2 Gy and 0.5 Gy when compared to the control cells and cells that received 0.1 Gy. However, Scheeffes' test assigned significance only to the differences established between the cells that received 0.5 Gy and the other groups of lymphocytes. .
Highlights
Vet. glasnik 63 (5-6) 341 - 352 (2009) Dunja Rukavina et al.: Chromosomal damage and mitotic index in Bosnian and Herzegovinian mountain horse lymphocytes following
DNA molecules are the target of ionizing irradiation and their damages are indubitably involved in the process of malignant transformation induced by irradiation
Mitotic index was determined as a relative frequency (%) of cells in mitosis per 1000 examined cells in 48-hour horse blood cultures irradiated with different dosages
Summary
HROMOZOMSKA O[TE]ENJA I PROMJENE MITOTSKOG INDEKSA U LIMFOCITIMA BOSANSKOHERCEGOVA^KOG BRDSKOG KONJA NAKON IN VITRO OZRA^IVANJA NISKIM DOZAMA X ZRAKA. In this study we investigated the influence of low dosage X - ray irradiation on the incidence of chromosomal damage and changes in mitotic index (MI) in cultured peripheral lymphocytes of the Bosnian and Herzegovinian mountain horse following in vitro irradiation. Our study was aimed at examining the correlation between the irradiation dosage and the number of chromosomal aberrations as well as alteration of mitotic index in Bosnian and Herzegovinian mountain horse lymphocytes following in vitro irradiation with a low dosage of X-rays. A total of 958 metaphases were examined in the samples irradiated with 0.5 Gy. The observed aberrations were statistically analyzed using variance analysis and post-hoc multiple tests (LSD test, Scheeffes' test) by SPSS 15.0 for Windows program. Mitotic index was determined as a relative frequency (%) of cells in mitosis per 1000 examined cells in 48-hour horse blood cultures irradiated with different dosages.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
