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Abstract
The four members of the Fos gene family give rise to proteins that are part of the AP-1 transcription factor complex. When studying cAMP-induced apoptosis in a leukemia cell line from rat, we found that theFra-2gene (coding for the Fos-related antigen-2) became strongly upregulated as the leukemia cells started to die. It was therefore of interest to determine the cytogenetic localization of the humanFra-2gene (FRA2), including a comparison to chromosomal aberrations observed in leukemia patients. Based on sequence information from the rat and chickenFra-2homologs, we were able to PCR-amplify a 4.5-kb genomic fragment covering exon 4 ofFRA2.This fragment was employed as probe for both radioactive and fluorescencein situhybridization to human metaphase chromosomes, allowing us to assignFRA2to 2p22–p23. The localization of the gene to chromosome 2 was independently verified by PCR amplification of aFRA2-specific fragment from a panel of rodent–human somatic cell hybrids.
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