Abstract
A study on the variation in chromosome number of the cells from a nodular morphogenic callus (Mohanty and Ghosh 1987), derived from embryo or mesocotyl, or leaf, was undertaken. This study also covered the extent of variation in relation to repeated subculturing for 6 months. The chromosome number showed wide variations (2n=5 to 100); the diploid cells (2n=2x=14), however, were dominant in all the cultures obtained from each type of explant. In an embryo callus, the tetraploid (2n=4x=28) cells increased significantly after 90 days of culturing, but no significant rhythmic increase of tetraploid cells with age could be observed in mesocotyl and leaf calli. The frequency of aneuploid and high polyploid cells decreased with increase in age of the culture.
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