Chromosomal aberrations in synchronized mammalian cells treated with 5-bromo-deoxyuridine and irradiated by ultra-violet light.

Abstract

RECENTLY 5-bromodeoxyuridine (BUDR) has been used extensively in irradiation investigations1–3, and its radiation-sensitizing action, together with its specific site of incorporation, has been used to support the contention that DNA is the primary site of radiation damage in cells4. BUDR alone affects chromosomal structure after prolonged treatment5, or at comparatively high concentrations6, but in less drastic and in a qualitatively different fashion than in cells irradiated with X-rays.