Abstract
Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1 (high pigment1 [hp1]), Deetiolated1 (hp2), Zeaxanthin Epoxidase (hp3), and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled with an increase in chloroplast number and size. However, little is known about the underlying mechanisms exaggerating the carotenoid accumulation and the chloroplast number in these mutants. A comparison of proteome profiles from the outer pericarp of hp1 mutant and wild-type (cv Ailsa Craig) fruits at different developmental stages revealed at least 72 differentially expressed proteins during ripening. Hierarchical clustering grouped these proteins into three clusters. We found an increased abundance of chromoplast-specific carotenoid-associated protein (CHRC) in hp1 fruits at red-ripe stage that is also reflected in its transcript level. Western blotting using CHRC polyclonal antibody from bell pepper (Capsicum annuum) revealed a 2-fold increase in the abundance of CHRC protein in the red-ripe stage of hp1 fruits compared with the wild type. CHRC levels in hp2 were found to be similar to that of hp1, whereas hp3 and Ip showed intermediate levels to those in hp1, hp2, and wild-type fruits. Both CHRC and carotenoids were present in the isolated plastoglobules. Overall, our results suggest that loss of function of DDB1, DET1, Zeaxanthin Epoxidase, and Ip up-regulates CHRC levels. Increase in CHRC levels may contribute to the enhanced carotenoid content in these high-pigment fruits by assisting in the sequestration and stabilization of carotenoids.
Highlights
Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein1, Deetiolated1, Zeaxanthin Epoxidase, and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled with an increase in chloroplast number and size
In this study, using a proteomic approach, we examined the influence of DNA Damage-Binding Protein1 (DDB1) on tomato fruit ripening
We found an additional role for DDB1 and DET1 in up-regulating the level of chromoplast-specific carotenoid-associated protein (CHRC) by 2fold by an unidentified mechanism
Summary
Tomato (Solanum lycopersicum) high-pigment mutants with lesions in diverse loci such as DNA Damage-Binding Protein (high pigment1 [hp1]), Deetiolated (hp2), Zeaxanthin Epoxidase (hp3), and Intense pigment (Ip; gene product unknown) exhibit increased accumulation of fruit carotenoids coupled with an increase in chloroplast number and size. Tomato (Solanum lycopersicum) high-pigment mutants hp (hp, hp1w), hp (hp, hp2j, hp2dg), hp, and Intense pigment (Ip) share characteristically high lycopene content and exhibit an increased number and size of chloroplasts in leaves as well as in green fruits. This leads to a higher number of chromoplasts when the fruits ripen (Azari et al, 2010). In all the above cases, the efficient conversion of chloroplasts to chromoplasts is necessary to accumulate the high amount of synthesized carotenoids
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have