Abstract
Chromocult Coliform agar and duplex PCR assays as methodologies for tracking Escherichia coli K12 in industrial biotechnological processes
Highlights
The technology of manufacturing biopharmaceuticals and other biomolecules can be divided into upstream and downstream processes
Chromogenic Agar (CCA) supplemented with ampicillin was used in experiments of intentional contamination with E. coli K12 and verification of recovery rate in medium
The results confirmed the efficiency of the Killing System and Germ Filtration in ensuring that residuals and final product derived from recombinant DNA technology are free of GMOs
Summary
The technology of manufacturing biopharmaceuticals and other biomolecules can be divided into upstream and downstream processes. According to Brazilian legislation, the risks and effects of upstream and downstream biotechnological processes arising from the release of GMOs into the environment must be evaluated. In this sense, research institutions and industries active in the biotechnology field must develop methods for the detection of GMOs in developed processes, in order to identify them in case of accidental release to the environment (Miralles et al, 2009; Costa et al, 2011; Simões et al, 2016)
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