Abstract
Some emerging studies have suggested that chromobox homolog 8 (CBX8) may play a critical role in carcinogenesis and prognosis in human cancer. Based on The Cancer Genome Atlas (TCGA)’s available data and the Gene Expression Omnibus (GEO) database, we conducted a systematic analysis of the carcinogenic effects of the CBX8 gene. We used TIMER2, GEPIA2, UALCAN, cBioPortal, Kaplan-Meier plotter, OncoLnc, STRING, HPA, and Oncomine data analysis websites and R data analysis software to analyze available data. The results show that the level of expression of CBX8 was significantly different among 27 different types of tumors and adjacent normal tissues. Moreover, we found that CBX8 expression had a close relationship with prognosis in some kinds of cancers. The phosphorylation level of some protein sites (such as S256) was significantly increased in tumors. CD8 + T-cell, B-cell and cancer-associated fibroblast infiltration levels were associated with CBX8 expression. The results of enrichment analysis indicated that the main biological activities of CBX8 are connected to gene transcription and repair of DNA damage. In conclusion, the level of expression of CBX8 was closely related to carcinogenesis and prognosis of some kinds of tumors, which needs further experimental verification.
Highlights
Chromobox homolog 8 (CBX8), called Human Polycomb 3, is an important member of Polycomb Group (PcG) proteins, which were first reported as critical developmental regulators in Drosophila (Benetatos et al, 2014; Chiacchiera et al, 2016)
We further evaluated the differences in the expression of chromobox homolog 8 (CBX8) between the normal tissues and tumor tissues of uterine carcinoma (UCS), acute myeloid leukemia (LAML), ovarian serous cystadenocarcinoma (OV), lymphoid neoplasm diffuse large B-cell lymphoma (DLBC), brain lower grade glioma (LGG), skin cutaneous melanoma (SKCM), testicular germ cell tumors (TGCT), and thymoma (THYM) (Figure 1B, all P-values < 0.01)
Through analysis of the Clinical Proteomic Tumor Analysis Consortium (CPTAC) dataset, we found that CBX8 protein had higher expression in breast cancer, clear cell renal cell carcinoma (RCC), colon cancer, lung adenocarcinoma (LUAD), ovarian cancer, and uterine corpus endometrial carcinoma (UCEC) (Figure 1C, all P-value < 0.001) than in related normal tissues
Summary
Chromobox homolog 8 (CBX8), called Human Polycomb 3, is an important member of Polycomb Group (PcG) proteins, which were first reported as critical developmental regulators in Drosophila (Benetatos et al, 2014; Chiacchiera et al, 2016). As an important factor involved in cell life activities, CBX8 inhibits the transcription of some critical target genes, including the INK4a/ARF gene (a tumor-suppressing gene affecting the survival of cells) (Dietrich et al, 2007) and the AF9 gene (related to acute leukemia occurrence) (Tan et al, 2011), and can regulate cell differentiation (Tang B. et al, 2019). A link between CBX8 and tumors has been consistently found. The overexpression of CBX8 indicates a poor prognosis (Tang B. et al, 2019).
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