Chromium uptake and transport in barley seedlings (Hordeum vulgare L.)

Abstract

Potassium chromate is more toxic to the growth of barley in solution culture than chromic chloride, though apparent uptake of the latter is much faster. Inhibitor studies indicate that CrO4 (2-) uptake is "active" whereas Cr(3+) uptake is passive, demonstrating that the two forms do not share a common uptake mechanism. Studies on the form of Cr inside root cells show that in plants fed CrO4 (2-) the Cr remains largely unchanged whereas in plants fed Cr(3+) a little CrO4 (2-) (0.5 per cent) is produced. This conversion is dependent on the presence of living material and is probably enzymatic. Chromate uptake follows Michaelis-Menten kinetics at low concentration and is competitively inhibited by sulphate. Transport of chromium up the root is very slow, accounting for the low levels of Cr in the shoots. Chromate is transported better than Cr(3+) though still to a very limited extent. These experiments provide a physiological basis for previous observations.