Chromium [Cr(VI)] biosorption property of the newly isolated actinobacterial probiont Streptomyces werraensis LD22.

S Latha,G Vinothini,D Dhanasekaran

doi:10.1007/s13205-014-0237-6

Abstract

The present work demonstrates the heavy metal resistance and detoxification of Cr(VI) by the probiotic actinobacterial cultures isolated from chicken and goat feces. The actinobacterial isolates were screened for heavy metal resistance by qualitative, semiquantitative assays and Cr(VI) biosorption was determined by analytical techniques such as atomic absorption spectrophotometry and Fourier transform infrared spectrometry (FT-IR). All the tested actinobacterial isolates (n = 20) showed resistance toward K2Cr2O7, NiCl2, ZnCl2, CuSO4 and PbNO3 at 20 mg L−1 concentration. The maximum tolerance concentration values were found to be 200–250 mg L−1 for K2Cr2O7, 100–250 mg L−1 for PbNO3 and <50–250 mg L−1 for NiCl2, ZnCl2 and CuSO4. Among the five tested heavy metals, Cr(VI) was resisted by 95 % of the tested actinobacterial cultures up to 250 mg L−1 concentration; particularly, the isolate LD22 exhibited a high degree of tolerance to all the tested heavy metals. Thus, the isolate was justifiably chosen for Cr(VI) biosorption study and the biosorption efficacy was found maximum at 100 mg L−1 of metal ion concentration (3 g L−1 of biomass dosage and pH 7.0). FT-IR spectrum revealed the chemical interactions between the hydroxyl, amine and carboxyl groups of the biomass and the metal ions. On the basis of phenotypic, physiological, biochemical and molecular characteristics the isolate LD22 was identified as Streptomyces werraensis LD22 (JX524481) which could be used to develop a biosorbent for adsorbing Cr(VI) metal ions.Electronic supplementary materialThe online version of this article (doi:10.1007/s13205-014-0237-6) contains supplementary material, which is available to authorized users.

Highlights

Heavy metals are widespread pollutants of great concern as they are non-biodegradable and persistent in nature
The actinobacterial isolates were screened for heavy metal resistance by qualitative, semiquantitative assays and Cr(VI) biosorption was determined by analytical techniques such as atomic absorption spectrophotometry and Fourier transform infrared spectrometry (FT-IR)
All the 20 isolates showed a clear visible growth in the minimal medium agar supplemented with the metals K2Cr2O7, NiCl2, CuSO4, Atomic absorption spectroscopy (AAS) is one of the important analytical techniques used to ascertain the concentration of metal ions in a solution

Summary

Introduction

Heavy metals are widespread pollutants of great concern as they are non-biodegradable and persistent in nature. They occur naturally in the environment, but their concentrations are frequently elevated as a result of pollution introduced into the environment through different human activities such as mining, smelting, sewage sludge disposal, application of pesticides, inorganic fertilizers and atmospheric emissions (Liu et al 2005). All mineral elements, whether considered to be essential or potentially toxic, can have an adverse effect upon humans and animals if included in the diet at excessively high concentrations (Okoye et al 2011).

Methods

Results

Conclusion