Abstract
Methods have been developed for the chromato-colorimetric determination of tselanid (lanatoside C) as such and in tablets and solutions. The chloroform-methanol-water (80:19:1) solvent system was used. Chromatography was performed by the ascending method on Silufol UV-254 plates. The revealing agent was a 1% solution of vanillin in 10% perchloric acid. Quantitative determination was performed photocolorimetrically by the reaction with sodium picrate. The methods developed have given accurate results correlating with those of high-performance liquid chromatography and the biological method. The relative error of this determination does not exceed ±4% [1].
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