Abstract
The ion-exchange method of W hite and H ess for the urine desalting and its several modifications were employed as a model to study some factors important for the preparation of high quality sugar concentrate for paper chromatography. The main investigated features included the urine:resin ratio; desalting by passage through columns vs. by mixing and shaking; and the effect of acetate and OH forms of the anion-exchange resin on saccharides. At least three times more urine may be desalted with the W hite and H ess system composed by Amberlite IR 120 (H form) and IRA 410 (acetate form) than had been previously described. These resins or their monobed mixture Amberlite MB-3 (acetate form) gave the best results as demonstrated by high yield and good reproducibility in both colorimetric and enzymatic determinations of sugars and qualitative paper chromatography, using volumes of urine as much as fifty times greater than those proposed in the original work. The quality of desalting is most sensitively indicated by the levels of urea. Sensitive but less reliable are fluorescing urinary pigments. Inorganic salts are more readily removed than other interfering substances.
Published Version
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