Abstract
THE extreme ease of oxidation of the thiol group has hindered the chromatographic separation of mercaptans. Hanes, Hird and Isherwood1 overcame this difficulty by combining the thiol group with N-ethyl maleimide before chromatographic separation. Cysteine and glutathione were separated in this way and detected by spraying the developed papers with ninhydrin. This spray is not specific for mercaptans, however, and Price and Campbell2 expanded the technique by condensing the mercaptans with N-(4-hydroxy-1-naphthyl) isomaleimide and, after chromatographic separation, detecting the naphthyl derivative by spraying with tetra-azotized di-o-anisidine.
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