Abstract
A mathematical model is developed to describe the chromatographic behavior of a reversibly reacting mixture of two linearly adsorbed components. When the distribution coefficient between the fluid and the solid phases is different for the two components, the elution profiles are broadened to an extent that depends upon the rate of reaction. For low reaction rates, the two components behave independently and two separate peaks are obtained when an equilibrium mixture is injected. At intermediate reaction rates, the elution profiles are stretched between the characteristic elution times of the two components. Finally, at high reaction rates, the two components propagate through the column as an equilibrium mixture and two superimposed symmetrical peaks are obtained. The mathematical development is used in the analysis of experimental results for the chromatographic separation of glucose—fructose mixtures at diferent temperatures. Each component peak is broadened and distorted by the simultaneous occurrence of mutarotation, which results in the formation of differently sorbed tautomers for each sugar. The model developed allows a determination of equilibrium and rate constants for each tautomer and a successful prediction of the chromatographic behavior.
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