Abstract
A variety of modifications of the Bensley and Bensley method for the preparation of a fat-staining solution from commercial Sudan III were examined for dye content by paper chromatography and for fat staining efficiency on frozen sections of Musca domestica larvae. These modifications have led us to believe that the most effective staining agent in commercial Sudan III is a bright orange compound with an Rf value of 0.92 in an isooctane system. This compound could be obtained in good yields by reflux extraction of the commercial dye widi dry petroleum ether (1 g. dye to 100 ml. solvent) for 2 hours, filtering, concentrating to 25 ml., and adding the concentrated solution to a 25 × 200 mm. Celite-silicic acid column. The chromatogram was then developed with petroleum ether for 3 hours, dried by suction and extruded. The orange band was sectioned, air dried and stored. One gram of this dry powder extracted with 10 ml. of 40% alcohol gave an efficient staining solution.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
