Chromatography, Affinity

Abstract

Abstract Affinity chromatography is a liquid chromatographic technique that uses a biologically related ligand for the specific retention or analysis of a given target. The general theory of affinity chromatography is considered and several examples are given of its typical applications. A number of items to consider in the use of this method are discussed, including the choice of ligand, support material, immobilization method, and solvent conditions. Several types of affinity chromatography are then examined in more detail. Particular attention is given to the techniques of bioaffinity chromatography, immunoaffinity chromatography, boronate affinity chromatography, immobilized metal‐ion affinity chromatography, dye‐ligand affinity chromatography, and biomimetic affinity chromatography. The use of affinity chromatography in the measurement of thermodynamic and kinetic constants is also considered, as well as the use of this method in sample pretreatment, purification, and analysis. Other items that are discussed include recent developments in the use of molecular imprints as affinity ligands and the use of biosensors for the study of biological interactions.