Abstract

Conditions for the chromatography of amino acyl-acceptor RNA on paper sheets of DEAE-cellulose have been explored further. Amino acid-specific RNA's can be separated using this type of chromatography. It was found that significant amounts of RNA are bound to the paper so that (I) salt solutions were ineffective eluents of RNA, and (2) urea combined with salt was effective. Results suggest that non-electrostatic forces play a significant role in the adsorption of RNA to substituted cellulose.

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