Abstract

Apolar ecdysteroid conjugates (acetates and long-chain fatty acyl esters) are being identified from a wide variety of invertebrate sources. It is apparent that they form a major class of ecdysteroid conjugates which can serve either as inactivation products prior to excretion or as a storage form of ecdysteroid. We have assessed the suitability of reversed-phase (RP) high performance liquid chromatography (HPLC) on a Cis-stationary phase and normal phase (NP) HPLC on silica for the separation and identification of such conjugates. When used in conjunction, these systems provide adequate resolution and selectivity for the preliminary identification of ecdysteroid acetates and fatty acyl esters.

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