Abstract
ABSTRACT The usefulness of partition chromatography on cellulose columns has been tried in fractionation of urinary corticosteroids. As the chromatographic system toluene/propylene glycol has been used. During the first part of the experiments the determination of the corticosteroid content in the chromatographic fractions was carried out by measuring their reducing power against phosphomolybdic acid. Provided the right conditions were used, a satisfactory separation was obtained of the following steroids in mixture: 11-deoxycorticosterone, 11-dehydrocorticosterone, 11-deoxy-17-hydroxycorticosterone, corticosterone, cortisone, dihydrocortisone, tetrahydrocortisone, cortisol and tetrahydrocortisol. Aldosterone appeared in the same fractions as cortisone. By means of the borohydride-bismuthate reaction and a correction by the Allen formula in the subsequent spectrophotometry, an approximatively specific determination of a series of 17-hydroxycorticosteroids in urine extracts could be obtained.
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