Chromatographic separation of unsaturated fatty acids as their 2,4‐dinitrobenzenesulfenyl chloride derivatives

Abstract

SummaryOleic, linoleic, and linolenic acids were found to react with 2,4‐dinitrobenzenesulfenyl chloride to form addition products of constant composition. Saturated acids did not react. The derivatives of mixed acids were separable into well defined bands on MgSO4 columns and recoverable as individual compounds in the eluate, with yields above 95% in each case.This technique provided the basis for a new method of analysis, which we have called the “isolation” method. Results obtained in the analysis of 100‐mg. samples of common vegetable oils compared favorably with those obtained by the spectral absorption method except that isolation values for saturated acids were lower.