Abstract
The chromatography of polar compounds, such as coumarins and flavonoids, has been investigated on diol-bonded silica gel layers using various organic modifiers (2-propanol (2PrOH), ethyl acetate (EtOAc), methyl ethyl ketone (MeEtCO), 2-propyl ether (2Pr)2O, 1,4-dioxane (DX) and tetrahydrofuran (THF)) in n-heptane as the mobile phase. It was found that diol phases are very suitable for separation of the above-mentioned compounds in normal phase thin-layer chromatography. The selectivity of the systems, the adsorptive properties of the diol-modified phases, and the influence of the organic modifiers on retention are discussed. Some results, obtained on diol-bonded silica gel, are compared to underivatized silica gel as RM diol vs. RM silica relationship.
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