Abstract
Similar immunoglobulin (Ig) classes were obtained from porcine colostral whey by either column or batch chromatographic procedures; a stepwise buffer elution technique was used. Specific transmissible gastroenteritis virus neutralizing antibody was found in the 4 major fractions eluted comprising of IgG 1, IgG 2, IgA, and IgM. The IgG 1 and IgG 2 were essentially homogeneous, and the IgA- and IgM-rich fractions had to be recycled several times through Sephadex G-200 to obtain pure IgA and IgM that had specific virus neutralizing activities per mg of protein of 342.1 and 302.4, compared with 7.6 for IgG. By a combination of the batch chromatographic procedure and gel filtration, gram amounts of specific Ig could be fractionated from the same colostrum.
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