Abstract
Similar immunoglobulin (Ig) classes were obtained from porcine colostral whey by either column or batch chromatographic procedures; a stepwise buffer elution technique was used. Specific transmissible gastroenteritis virus neutralizing antibody was found in the 4 major fractions eluted comprising of IgG 1, IgG 2, IgA, and IgM. The IgG 1 and IgG 2 were essentially homogeneous, and the IgA- and IgM-rich fractions had to be recycled several times through Sephadex G-200 to obtain pure IgA and IgM that had specific virus neutralizing activities per mg of protein of 342.1 and 302.4, compared with 7.6 for IgG. By a combination of the batch chromatographic procedure and gel filtration, gram amounts of specific Ig could be fractionated from the same colostrum.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.