Abstract
A column chromatographic method for the separation of dansyl amino acids and dansyl amines is described in which Amberlite IRC-50 equilibrated with the eluent is used as the stationary phase and six solvent mixtures, which are composed of a buffer or 2 % acetic acid and tetrahydrofuran, methyl ethyl ketone and acetone, are used as eluents. Neutral and acidic dansyl derivatives were separated at low pH, and those having a positive charge or present as the dipolar ion at pH 5.60 were separated at high pH. Quantitative determination of histamine as its monodansyl derivative was successful, and an analysis for histamine in a rat liver extract is described.
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