Abstract
HPLC (High Performance Liquid Chromatography) was utilized for the chiral separation of racemic bupivacaine, and mathematical modeling with competitive Langmuir isotherm was performed to determine the opti- mum feed condition. For each racemic compound, the isotherm parameters a, b and mass transfer coefficients k were obtained by parameter estimation and maximum likelihood method. The agreement of elution profiles between the experimental data and the calculated values was fairly good. In order to find the optimum separation condition, simulations were carried out to determine the feed conditions such as concentration and injection volume. To preparatively separate racemic bupivacaine, the desirable injection volumes were 0.05 ml at 2.0 mg/ml of the con- centration of racemic mixture or 0.01 ml at 20 mg/ml.
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