Abstract
Type I collagen (PSC-I) and type V collagen (PSC-V) were directly separated by DEAE-cellulose chromatography without salt precipitation from the skin of deep-sea redfish after limited pepsin digestion. The yields of PSC-I and PSC-V were 79.4% and 8.4%, respectively. The denaturation temperature of PSC-V (17.8°C) was significantly higher than that of PSC-I (15.9°C), as a result of its higher total content of imino acid (17.1%) and hydroxylation degree of proline and lysine (44.7%) than those of PSC-I (14.7% and 35.8%). However, the intrinsic viscosity and viscosity transition temperature span of PSC-I (14.4dL/g and 8°C) were considerably higher than those of PSC-I (13.5dL/g and 5°C), which could be attributed to its greater extent of triple helix and more intermolecular crosslinks as shown by SDS-PAGE pattern and infrared spectra.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
