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Abstract
The gentamicin complex consists of three components, C1, C1a, and C2, which are separated by paper chromatography using the lower phase of a solvent system of chloroform, methanol, and 17% ammonia (2:1:1). Two identical chromatograms are developed. On one, the positions of these components at Rf's of approximately 0.75, 0.35, and 0.50, respectively, are located by means of ninhydrin. On the other, corresponding areas are cut out, eluted from the paper with 0.1 M potassium phosphate buffer, pH 8.0, and assayed microbiologically. This procedure determines the relative amounts of the three gentamicin fractions, and is superior to the original official FDA procedure that measured only two fractions.
Published Version
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