Abstract
AbstractThe application of chromatography represents an increasing trend in the field of virus purification. This article focuses on the unique constraints imposed by virus particles and how they relate to the strengths and limitations of various chromatography media. Specific attention is given to the contributions of different types of physical supports, including porous particles, monoliths, and adsorptive membranes. Media characteristics are also discussed in the context of purification process control, reproducibility, and process economics. Protocols are offered for conducting method development with size exclusion, ion exchange, hydrophobic interactions, hydroxyapatite, and bioaffinity chromatography, and guidelines are summarized for combining individual methods into integrated multistep purification procedures suitable for preparation of clinical material.
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