Abstract
Abstract A method is described for the quantitative determination of reserpine in tablet formulations. A suitable amount of tablet material is triturated with dimethylsulfoxide (DMSO), incorporated onto Celite, and transferred to a chromatographic column containing acid, base, and water layers. The reserpine is eluted with chloroform and quantitated spectrophotometrically. Duplicate results from 16 collaborators averaged 100.7% recovery as determined by UV, 98.8% recovery determined by colorimetric analysis. The method is recommended for adoption as official first action.
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