Abstract
Object: The present study was aimed to assess the anti‑arthritic activity of chloroform fraction of Alstonia scholaris leaf extract against Freund’s complete adjuvant (FCA)‑induced arthritis in rats. Materials and Methods: The anti‑inflammatory activity of various fractions of ethanolic extract of Alstonia scholaris at concentration of 100 mg/kg was studied using the carrageenan‑induced inflammatory models. The chloroform fraction shows significant anti‑inflammatory activity. The chloroform fraction was further studied for anti‑arthritic activity and HPTLC fingerprint analysis. For anti‑arthritic activity, the active chloroform fraction was administered at the concentrations of 50 and 100 mg/kg body weight. The effect of chloroform fraction on liver ALP, ACP and LDH levels of lysosomal enzymes of FCA arthritic animals were studied. Indomethacin and prednisolone (10 mg/kg) was used as standard. HPTLC studies were carried out using CAMAG HPTLC system equipped with linomat IV applicator, TLC scanner; Reprostar 3 and WIN CATS‑4 software were used. Results: The chloroform fraction at 100 mg/kg, showed maximum inhibition (34.16%) of inflammation induced by carrageenan. In FCA‑induced arthritis, the chloroform fraction showed a highly significant reduction in paw volume (50 mg/kg-72.71%; 100 mg/kg-74.35%). The levels of lysosomal enzymes were significantly decreased in the chloroform fraction‑treated groups. Conclusion: The possible mechanism of action of the chloroform fraction of Alstonia scholaris leaf extract may be through its stabilising action on lysosomal membranes. Future studies will provide new insights into the anti‑arthritic activity of Alstonia scholaris and isolation of compound from it may eventually lead to development of a new class of anti‑arthritic agent. Key words: Alstonia scholaris, athritis, chloroform fraction, lysosomal membrane stabilisation
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