Abstract
Fractionation of ultrafiltrates of rat hypothalamic extracts has been performed on Sephadex LH-20 in dimethylformamide/water. The fractions were examined for their content of luteinizing hormone-releasing activity by bioassay in vitro utilizing LH radioimmunoassay, and also for their content of LRF decapeptide-like material by direct radioimmunoassay. Each method revealed the same pattern of activity. This consisted of a major peak whose elution position coincided with that of authentic decapeptide, and a minor region which closely preceded it, often taking the form of a shoulder on the leading edge of the main peak. The active component in the minor peak could be completely resolved by repeated filtration in the same system thus indicating that it represented another gonadotropin-releasing species rather than a chromatographic artefact although its biological and immunological activities indicate that its structure must resemble closely that of the decapeptide.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
