Abstract
A method is presented for the determination of 4,4′-methylenedianiline in hydrolysed human urine, based on a derivatization procedure with pentafluoropropionic anhydride and capillary gas chromatographic determination with selected ion monitoring. Dideuterated 4,4′-methylenedianiline was used as an internal standard. The ratio of the molecular ion of the 4,4′-methylenedianiline amide derivative ( m/z 490) and the dideuterated 4,4′-methylenedianiline amide derivative ( m/z 492) was used for quantitative analysis. The calibration graph was linear in the investigated range (5–30 μg/l in urine) with a correlation coefficient of 0.998. The precision was 3.4% for urine samples containing 26 μg/l and the detection limit was 2 μg/l. The overall recovery for urine samples containing 115 μg/l was 96 ± 5%. Urine samples from eight skin-exposed epoxy resin workers were examined and 4,4′-methylenedianiline was found in the concentration range 2–600 μg/l.
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