Chromatographic classification and comparison of commercially available reversed-phase liquid chromatographic columns containing polar embedded groups/amino endcappings using principal component analysis

Abstract

Polar embedded phases have become increasingly popular in liquid chromatography (LC) analysis. These phases can produce diverse chromatographic selectivities as a result of their differing base silica, the type of polar embedded group (i.e. amide, urea, carbamate, ether or sulphonamide moieties) and the length of the alkyl ligand. Four column characterization protocols, using differing test probes, have been used to characterize 18 of these phases together with 17 alkyl phases (some of which contained novel polar endcapping, i.e. amino), which have been evaluated using principal component analysis (PCA). PCA provided graphical comparisons of the differences/similarities between these phases and between their corresponding C-alkyl, amino endcapped and enhanced polar selectivity phases.