Abstract

Abstract The chromatographic behaviour of aromatic acids on an anion exchange resin was examined in order to develop a high-speed separation procedure of urinary aromatic acids. The ion-exchange resin was a macro porous anion-exchange resin (5μm particle size). The eluent was a mixture of an ammonium acetate buffer, acetonitrile and octylsodium sulfate. The concentration effect of acetonitrile, ammonium acetate buffer, octylsodium sulfate and temperature effect were discussed. The application of the present chromatographic system to urine analysis was also described.

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