Abstract
The combination of affinity chromatography and immunoassay provides a novel method for the quantitation of steroids in biological fluids. Anti-digoxin and anti-oestriol-16α-glucuronide antibodies were immobilised to cellulose strips, and used for ascending paper chromatography. Labelled digoxin and oestriol-16α-glucuronide were selectively retarded on strips containing the appropriate immobilised antibodies. The degree of retardation was quantitatively affected by the addition of non-labelled steroid to the sample. Calibration curves could, therefore, be constructed. The sensitivity of the assay system can be altered by changing the concentration of immobilised antibody on the cellulose strip. Calibration curves over the range 50–800 pg for digoxin and 50–250 pg for oestriol-16α-glucuronide are presented.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
