Abstract
The separation of enantiomers of some chiral weak acids was studied in HPLC with chiral HPLC columns prepared by coating or covalent immobilization of the same chiral selector, namely amylose tris(3-chloro-5-methylphenylcarbamate) onto silica. After screening some representatives of arylpropionic acid derivatives, coumarins and barbiturates in hydrocarbon-alcohol type mobile phases, we studied the temperature dependence of separation parameters for ketoprofen and naproxen. Instances of reversal of the enantiomer elution order were observed function of column temperature, nature of polar modifier and its content in the mobile phase, as well as between the coated and covalently immobilized versions of the columns made with more-or-less the same chiral selector. Thermodynamic parameters such as Gibb’s free energy, the standard molar entropy and the standard molar enthalpy of analyte transfer from the mobile to the stationary phase were calculated in some cases in order to explain the differences observed in the enantiomer separation ability and pattern of coated and covalently immobilized columns.
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