Abstract
Abstract A review is presented of chromatographic procedures used in the assay of vitamin C. Paper, thin layer, and column chromatography have been used to advantage for separating interfering substances. Even the closely related erythorbic acid, which has no vitamin C activity, can be separated from ascorbic acid by various techniques on paper. Total vitamin C can be determined chromatographically after reduction of dehydroascorbic acid with H2S or after oxidation of the reduced form to dehydroascorbic acid. A combination of column and thin layer chromatography on silica gel of the dinitrosazone formed by reaction of dehydroascorbic acid with 2,4-dinitrophenylhydraiine is recommended as an effective method for achieving specificity in vitamin C assays of foods and feeds where the level of interference is high. Efficient extraction procedures are described for determining added ascorbic acid in feeds and mineral premixes.
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