Chromatographic analysis (LC-MS and GC-MS), antioxidant activity, antibacterial activity, total phenol, and total flavonoid determination of Cleome arabica L. growing in Jordan

Abstract

ABSTRACT The pivotal Cleome arabica L. species belongs to the Cleome genus with fascinating secondary metabolites consisting of main classes of phenols, flavonoids, and glucosinolate derivatives. The current study was carried out on three crude extracts of Cleome arabica L. plant of Jordanian origin. In terms of the valuable resources for natural new drug development. The phytochemical contents, antioxidants, and antibacterial activities, in addition to the oil composition of this species were identified. Forty-six compounds representing 96.98% of the total composition were detected from the essential oil obtained by hydrodistillation (0.06%). The oil composition was dominated by nitrogen-sulfur-containing compounds (35.66%), esters (19.64%), and oxygenated sesquiterpenes (11.92%). Butanol extract had significantly the best scavenging activities of DPPH (P < .0001), ABTS (P < .0001), and hydroxyl radicals (P < .0001) while the Aq.MeOH extract exhibits the highest flavonoid content (173.5 ± 0.002 mg Quercetin/g dry extract) and had significantly the most ferrous chelating effect (P = .0011). Also, most of the tested extracts showed antibacterial activities against bacterial strains. Our findings exhibited that the Cleome arabica L. plant from Jordan origin might be a good candidate as potential biologically compounds that could be used as a source of natural antioxidants and antibacterial agents.