Abstract
The authors use Moore's autoradiographic method to study template activity of cerebellar cortical cells. Purkinje cells and granule cells from the cerebellar cortex of rats were used. A mixture of Tris-HCl buffer 100, sucrose 150, ammonium sulfate 80, 2-mercaptoethanol 12, /sup 3/H-UTP 0.02, unlabeled triphosphates 0.6 of each, MgCl/sub 2/ 8, and MnCl/sub 2/ 2 was used.
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