Abstract
Chromatin remodeling complexes (CRCs) use ATP hydrolysis to maintain correct expression profiles, chromatin stability, and inherited epigenetic states. More than 20 CRCs have been described to date, which encompass four large families defined by their ATPase subunits. These complexes and their subunits are conserved from yeast to humans through evolution. Their activities depend on their catalytic subunits which through ATP hydrolysis provide the energy necessary to fulfill cellular functions such as gene transcription, DNA repair, and transposon silencing. These activities take place at the first levels of chromatin compaction, and CRCs have been recognized as essential elements of chromatin dynamics. Recent studies have demonstrated an important role for these complexes in the maintenance of higher order chromatin structure. In this review, we present an overview of the organization of the genome within the cell nucleus, the different levels of chromatin compaction, and importance of the architectural proteins, and discuss the role of CRCs and how their functions contribute to the dynamics of the 3D genome organization.
Highlights
Reviewed by: Nataliya Soshnikova, Institute of Gene Biology (RAS), Russia Giovanni Messina, Sapienza University of Rome, Italy
We present an overview of the organization of the genome within the cell nucleus, the different levels of chromatin compaction, and importance of the architectural proteins, and discuss the role of Chromatin remodeling complexes (CRCs) and how their functions contribute to the dynamics of the 3D genome organization
These data arise the question if in organisms like Drosophila or plants, where the orthologs of CCCTC-binding factor (CTCF) do not seem to have an essential role at topologically associating domain (TAD) boundaries or where CTCF is totally absent, CRCs play an important role in directing architectural proteins to their target sites in order to control chromatin looping and TAD formation
Summary
Eukaryotic DNA is compartmentalized into hierarchically organized levels within the nuclear space. At the first level of compaction, in an interphase chromosome, there exists a 6.5 nm diameter cylinder-like structure called nucleosome, which is formed by histone octamers with 146 base pairs (bp) of DNA wrapped around this core in 1.6 turns (Felsenfeld and Groudine, 2003; Bassett et al, 2009; McGinty and Tan, 2015; Pombo and Dillon, 2015) This tetramer is formed by two heterodimers of the histones H3 and H4, which are flanked by two heterodimers of H2A and H2B histones in a structure known as the “histone core.”. The number of nucleosomes per clutch is variable; they are interspersed with nucleosomedepleted regions, and the nucleosome density is cell-type specific (Ricci et al, 2015)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
