Abstract
Here, we investigate the function of fission yeast Fun30/Smarcad1 family of SNF2 ATPase-dependent chromatin remodeling enzymes in DNA damage repair. There are three Fun30 homologues in fission yeast, Fft1, Fft2, and Fft3. We find that only Fft3 has a function in DNA repair and it is needed for single-strand annealing of an induced double-strand break. Furthermore, we use an inducible replication fork barrier system to show that Fft3 has two distinct roles at blocked DNA replication forks. First, Fft3 is needed for the resection of nascent strands, and second, it is required to restart the blocked forks. The latter function is independent of its ATPase activity.
Highlights
In eukaryotic chromosomes, the DNA is packaged into chromatin fiber structures to allow for compaction of DNA in the nucleus and proper chromosome segregation in mitosis and meiosis
Our work shows that of three Fun30 homologues, only Fft3 is implicated in the DNA damage repair in S. pombe
We show that Fft3 is required for proper single-strand annealing (SSA) and processing of arrested replication forks
Summary
The DNA is packaged into chromatin fiber structures to allow for compaction of DNA in the nucleus and proper chromosome segregation in mitosis and meiosis. Fun belongs to a subfamily of SNF2 enzymes with important roles in genome stability, gene regulation, and chromosome boundary function. Fft was recently implicated in transcription elongation by RNA polymerase II, where it is involved in disassembly and reassembly of nucleosomes to facilitate transcription (Lee et al, 2017). Another recent study found Fft in a genetic screen for factors needed for inheritance of heterochromatin at the silent mating-type loci. Fft was identified by a proteomic screen for proteins bound to a meiotic recombination hotspot and was shown to be one of several chromatin regulators required for efficient recombination at the ade6-M26 hotspot (Storey et al, 2018)
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