Abstract
The Simian Virus 40 (SV40) is composed of an outer shell formed from capsid proteins, enveloping a minichromosome that is made of double stranded DNA wound around ca. 20 nucleosomes. In contrast to many bacteriophages and RNA viruses, the structure and order of the packaged viral chromatin has remained elusive. Using small angle x-ray diffraction as well as computer modeling, we show that a unique ordering of the nucleic acid emerges, indicating at least two concentric shells of higher minichromosomal electron density. Analysis shows that packaging can be explained by considering the competition of interactions between disk-like nucleosomal particles that favor columnar ordering versus wall-nucleosome interactions that tend to align particles with the capsid interior.
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