Chromatin modified protein 4C (CHMP4C) facilitates the malignant development of cervical cancer cells.

Shu‐Li Lin,Qing Li,Mei Wang,Qing‐Qing Cao

doi:10.1002/2211-5463.12880

Abstract

Despite improvements in prevention and treatment, cervical cancer (CC) still poses a serious threat to women’s health. CHMP4C (chromatin modified protein 4C) is a subunit of the endosomal sorting complex required for transport, which is expressed in both nucleus and cytoplasm. Here, we examined the effect of CHMP4C on the biological behavior of CC cells and the underlying mechanisms. We report that CHMP4C expression is higher in CC tissues, and high CHMP4C expression is associated with lower survival. Up‐regulation of CHMP4C in C‐33A cells accelerates cell proliferation, migration and invasion, whereas down‐regulation of CHMP4C in Ca Ski cells had the opposite effect. Moreover, overexpression of CHMP4C induced activation of the epithelial–mesenchymal transition pathway, whereas depletion of CHMP4C inhibited activation. Our results suggest that CHMP4C contributes to the viability and motility of CC cells by modulating epithelial–mesenchymal transition and may facilitate the identification of novel biomarkers for CC therapy.

Highlights

Despite improvements in prevention and treatment, cervical cancer (CC) still poses a serious threat to women’s health
chromatin modified protein 4C (CHMP4C) is a subunit of the endosomal sorting complex required for transport, which is expressed in both nucleus and cytoplasm
This work aimed to verify the relevancy between the expression of CHMP4C and survival rate of patients with CC, and to explore whether the modulation of CHMP4C expression would impact the biological behavior of CC cells, as well as the regulatory pathways, hoping to discover a novel biomarker that can predict the development of CC

Summary

Introduction

Despite improvements in prevention and treatment, cervical cancer (CC) still poses a serious threat to women’s health. CHMP4C (chromatin modified protein 4C) is a subunit of the endosomal sorting complex required for transport, which is expressed in both nucleus and cytoplasm. Chromatin modified protein 4C (CHMP4C) belongs to the CHMP (chromatin modified protein) family It is a subunit of the endosomal sorting complex required for transport, which is expressed in both nucleus and cytoplasm [7,8]. Sadler et al [11] found that the polymorphism of CHMP4C increased the susceptibility of cancer and may induce tumorigenesis by disrupting the stability of genome. Abbreviations CC, cervical cancer; CCK-8, Cell Counting Kit-8; CHMP4C, chromatin modified protein 4C; EMT, epithelial–mesenchymal transition; HPV, human papillomavirus; qRT-PCR, quantitative real-time PCR; SD, standard deviation.

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