Abstract
Chromatin enrichment for proteomics (ChEP) is a technique that allows for unbiased proteomic profiling of the chromatin landscape using mass spectrometry. While the method has been successfully employed to survey chromatin-associated proteins in various organisms and cell types, ChEP has not yet been applied to plant materials. Here, we describe a detailed ChEP protocol which has been modified for plants and designated ChEP-P (ChEP in plants). The protocol outlined here includes all necessary steps to perform a label-free quantitative ChEP-P experiment, supporting the identification of more than 3500 proteins in Arabidopsis thaliana.
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