Abstract
C. vespertiliomis extracts were evaluated for antiproliferative and apoptosis effect on breast cancer (MCF7) cells. The leaves extracts were analysed for its antiproliferative effect on breast cancer (MCF7) cells and normal epithelial breast (MCF 10A) cells using Sulforhodamine B (SRB) assay. The selective extract was evaluated for its ability to induce apoptosis using Annexin V-FITC apoptosis staining and the expression of molecular genes using qualitative reverse transcription-polymerase chain reaction (RT-PCR) against MCF7 cells. Gas chromatography-mass spectrometry (GC-MS) was used to identify the compounds from the selective extract. The findings showed that dichloromethane fraction (CV-Dcm) extract had high antiproliferative effect against MCF7 cells (IC50 = 24µg/mL, selective index (SI) = 8.17). The percentages of apoptosis cells in CV-Dcm-treated MCF7 cells was 58.8%. The CV-Dcm extract induced downregulation of PCNA level. The apoptotic genes were also triggered in both extrinsic and intrinsic signaling pathways, affecting a 1.5-fold increase in BAX, 1.4-fold increase in cytochrome c, 1.3-fold increase in caspase-8, 1.7-fold increase in caspase-3 and 0.5-fold-decrease in BCL-2. Treated MCF7 cells also activated P53-dependent apoptotic death pathway. The present work strongly suggests that high efficacy of CV-Dcm extract was attributed to its antiproliferative and apoptosis-inducing activation in MCF7 cells, most likely due to its favourable compounds.
Highlights
Cancer is caused by rapid proliferation and development of abnormal cells [1]
The leaves extracts were analysed for its antiproliferative effect on breast cancer (MCF7) cells and normal epithelial breast (MCF 10A) cells using Sulforhodamine B (SRB) assay
The selective extract was evaluated for its ability to induce apoptosis using Annexin V-FITC apoptosis staining and the expression of molecular genes using qualitative reverse transcription-polymerase chain reaction (RT-PCR) against Michigan Cancer Foundation-7 (MCF7) cells
Summary
Cancer is caused by rapid proliferation and development of abnormal cells [1]. Global Cancer Incidence, Mortality and Prevalence (GLOBOCAN) (2020) reported that women breast cancer (11.7%) surpassed lung cancer (11.4%) as the most commonly diagnosed cancer [2]. F, which is a member of the Fabaceae family This plant is used to enhance blood circulation and treat snake bites, bone fractures, tuberculosis, colds and bronchitis. This plant is widely distributed in Southeast Asia and is native to China, Vietnam, Indonesia, Malaysia, Cambodia and Thailand [6]. [7] showed that C. vespertilionis extracts were effective against MCF7 cells. The objective of this study was to investigate the antiproliferative and apoptosis effect against treated MCF7 cells. C. vespertiliomis extracts were evaluated for antiproliferative and apoptosis effect on breast cancer (MCF7) cells
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have