Abstract
Chondroitin sulfate (CS) is an acidic polysaccharide comprised of repeating disaccharide units of N-acetylgalactosamine and glucuronic acid. It is known as a dietary supplement for joint articular cartilage care. CS is commonly extracted from bovine and shark cartilages by digestion of tissues with exogenous proteinase. This study was undertaken to extract CS from broiler chicken cartilage by tissue autolysis without using exogenous proteinase. Most (>90%) of tissue uronic acid was extracted by incubation with 0.5 mol/L sodium acetate at pH 4.5 and 37 °C overnight followed by tissue reextraction. There was no difference (P > 0.05) in the proportion of extractable uronic acid among cartilage tissues from different anatomical locations. The final product obtained after anion exchange chromatography was a large CS-peptide with glucuronosyl-N-acetylgalactosamine-4-sulfate as a predominant disaccharide. Keratan sulfate was detected by hexosamine analysis, susceptibility to keratanase, and immunodiffusion with anti-keratan sulfate monoclonal IgM. The CS-peptide also contained serine as a predominant amino acid accounting for approximately one third of total amino acid. It was concluded that tissue autolysis is a simple efficient procedure that can be scaled up for extraction of broiler chicken CS.
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