Chondroitin and glucosamine sulphate reduced proinflammatory molecules in the DRG and improved axonal function of injured sciatic nerve of rats

Olutayo Folajimi Olaseinde,Bamidele Victor Owoyele

doi:10.1038/s41598-022-06554-4

Olutayo Folajimi Olaseinde, Bamidele Victor Owoyele

Open Access

https://doi.org/10.1038/s41598-022-06554-4

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Journal: Scientific Reports	Publication Date: Feb 24, 2022
Citations: 2	License type: open-access

Affiliation: University of Ilorin

Abstract

Neuropathic pain (NP) is an abnormality resulting from lesion or damage to parts of the somatosensory nervous system. It is linked to defective quality of life and often poorly managed. Due to the limited number of approved drugs, limited efficacy and side effects associated with the approved drugs, drugs or drug combinations with great efficacy and very minimal or no side effects will be of great advantage in managing NP. This study aimed at investigating the synergistic antinociceptive effects of the combination of glucosamine sulphate (GS) (240 mg/kg) and chondroitin sulphate (CS) (900 mg/kg) in chronic constriction injury (CCI)-induced neuropathy in rats. Forty-two Wistar rats were randomly distributed into seven groups (n = 6). Sciatic nerve was ligated with four loose ligatures to induce NP. Effects of drugs were examined on stimulus and non-stimulus evoked potentials, expression of dorsal root ganglia (DRG) pain modulators and structural architecture of DRG. Oral administration of GS and CS for 21 days reduced hyperalgesia, allodynia, sciatic nerve functional aberration and DRG pain modulators. Histopathology and immunohistochemistry revealed restoration of structural integrity of DRG. Our result showed that the combination of GS and CS produced antinociceptive effects by attenuating hyperalgesia, allodynia and downregulation of NP mediators. GS and CS additionally produced synergistic analgesic effect over its individual components.

Highlights

Neuropathic pain (NP) is an abnormality resulting from lesion or damage to parts of the somatosensory nervous system
There were no significant differences between the baseline measurements in all the various tests prior to surgical intervention
glucosamine sulphate (GS) and chondroitin sulphate (CS) reversed cold allodynia. this study showed that cold allodynia characterised by increase in the duration of foot lift (DFL) to acetone drop test was observed in the ligated control (LC) animals and this was recognised from the 3rd day of constriction injury (CCI) intervention and significantly sustained till the 24th day of CCI intervention (Fig. 2A)

Summary

Introduction

Neuropathic pain (NP) is an abnormality resulting from lesion or damage to parts of the somatosensory nervous system. This study aimed at investigating the synergistic antinociceptive effects of the combination of glucosamine sulphate (GS) (240 mg/kg) and chondroitin sulphate (CS) (900 mg/kg) in chronic constriction injury (CCI)-induced neuropathy in rats. Most of the studies conducted in the systemic review were done in diabetic neuropathy or postherpetic neuralgia with no main focus on CCI of sciatic nerve. Reports have shown that drug combinations have given better results in the management of chronic neuropathic p ain[5]. Conducted to investigate the synergistic effect of the combination of chondroitin sulphate and glucosamine sulphate treatment on dorsal root ganglia inflammatory markers in CCI neuropathic pain in male Wistar rats

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