Chondrogenic Potential of Peripheral Blood Derived Mesenchymal Stem Cells Seeded on Demineralized Cancellous Bone Scaffolds.

Shao-Jie Wang,Dong Jiang,Hai-Jun Wang,Yan-Song Qi,Zheng-Zheng Zhang,Jia-Kuo Yu,Ai-Bing Huang,Ji-Ying Zhang

doi:10.1038/srep36400

Abstract

As a cell source with large quantity and easy access, peripheral blood mesenchymal stem cells (PBMSCs) were isolated and seeded in porcine demineralized cancellous bone (DCB) scaffolds, cultured in chondrogenic medium and evaluated for in vitro chondrogenesis. Bone marrow MSCs (BMMSCs) and articular cartilage chondrocytes (ACCs) underwent the same process as controls. The morphology, viability and proliferation of PBMSCs in DCB scaffolds were similar to those of BMMSCs and ACCs. PBMSCs and BMMSCs showed similar chondrogenesis potential with consistent production of COL 2 and SOX 9 protein and increased COL 2 and AGC mRNA expressions at week 3 but the COL 2 protein production was still less than that of ACCs. Minimal increase of hypertrophic markers was found in all groups. Relatively higher ALP and lower COL 10 mRNA expressions were found in both MSCs groups at week 3 than that in ACCs, whereas no significant difference of COL 1 and SOX 9 mRNA and MMP 13 protein was found among all groups. To conclude, PBMSCs shared similar proliferation and chondrogenic potential with BMMSCs in DCB scaffolds and could be an alternative to BMMSCs for cartilage tissue engineering. Further optimization of chondrogenesis system is needed regardless of the promising results.

Highlights

With the superiority of its less invasion and larger quantity for clinical settings, MSCs from peripheral blood (PBMSCs) have been isolated[7], and used as an alternative to Bone marrow MSCs (BMMSCs) to repair bone and cartilage regeneration in vivo[8,9]
The rabbit peripheral blood mesenchymal stem cells (PBMSCs) were seeded on porcine demineralized cancellous bone (DCB) scaffolds and the cell morphology, proliferation, and chondrogenic potential of the PBMSCs were accessed
The results of the present study found that a) PBMSCs displayed good cell viability and proliferation potential in a 3D environment; b) PBMSCs could differentiate into chondrocyte-like cells and form cartilage-specific matrix in 3D scaffolds with minimal expression of hyperthrophic markers; c) PBMSCs exhibited similar viability and chondrogenic potential in DCB scaffolds compared to BMMSCs; d) COL 2 production of PBMSCs or BMMSCs increased with time but was still less than that of early passage articular cartilage chondrocytes (ACCs) in DCB scaffolds

Summary

Introduction

With the superiority of its less invasion and larger quantity for clinical settings, MSCs from peripheral blood (PBMSCs) have been isolated[7], and used as an alternative to BMMSCs to repair bone and cartilage regeneration in vivo[8,9]. PBMSCs of rats and rabbits were identified and confirmed similar proliferation and multi-lineage differentiation potential compared to BMMSCs10,11. Those studies were mostly investigated in two dimensional monolayer culture which is significantly different from natural cell environment. The purpose of the present study was to explore the chondrogenic potential of the PBMSCs in demineralized cancellous bone (DCB), a commonly used 3D bioactive scaffold for cartilage tissue engineering. The rabbit PBMSCs were seeded on porcine DCB scaffolds and the cell morphology, proliferation, and chondrogenic potential of the PBMSCs were accessed. We hypothesized that the PBMSCs might show similar results to the controls regarding proliferation and chondrogenic potential in 3D microenvironment

Objectives

Methods

Results

Discussion

Conclusion