Abstract

Chondrogenic potential of hASCs expanded in flask or in a hollow-fiber bioreactor

Highlights

  • Articular hyaline cartilage has limited regenerative ability, and the loss of its function can frequently be painful and debilitating

  • Based on the weight of the information obtained by transmission electron microscopy (TEM) and their correlation with the other methodologies used in this study, we propose hollow-fiber bioreactor (HFBR) as valid and saving time method to expand Human adipose-derived stem/stromal cells (hASCs) without affect chondrogenic differentiation

  • Immunophenotyping was performed according to the International Society for Cell Therapy (ISCT) standards [18,35] to confirm the mesenchymal stem cells (MSCs) nature of the cells harvested after cell culture in flask or HFBR, i.e. before subjecting cells to chondrogenic differentiation

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Summary

Introduction

Articular hyaline cartilage has limited regenerative ability, and the loss of its function can frequently be painful and debilitating. Efforts have been made to intervene in cartilage defects with a regenerative medicine approach aiming at supporting biological repair with cell-based strategies for in situ regeneration of the tissue [1]. In this perspective, mesenchymal stem cells (MSCs) represent an appealing cell source for cartilage repair. Adipose tissue has gained increased interest as a source of MSCs because it is more accessible and can be obtained in large quantities by liposuction under local anesthesia with minimum discomfort for patients [14]

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