Abstract
Two potential biomarkers of pesticide contamination were investigated in Apporectodea caliginosa. Juvenile earthworms were exposed to sub-lethal concentrations of the organophosphates chlorpyrifos and diazinon to assess their effect on cholinesterase and glutathione S-trasferase activities. Cholinesterase which is inhibited by organophosphates, was inhibited by 72% by diazinon (P<0.001) and 87% by chlorpyrifos (P<0.001) within 1 day of exposure and remained depressed 14 days after exposure. Glutathione S-transferase (GST) is induced by xenobiotics compared to controls and diazinon induced GST by 56% after 14 days (P<0.001) and chlorpyrifos induced GST by 148% after 14 days exposure (P<0.005).
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