Abstract
Publisher Summary This chapter presents a summary of recent advances in choline acetyltransferase (ChAT) antibody development and immunohistochemical applications and discusses areas where there is general agreement and areas that will require further investigation. It focuses on studies describing localization of ChAT immunoreactivity. The accumulation of new knowledge about the function of acetylcholine in brain has been hampered by the lack of reliable methods for visualizing cholineric structures. There has been general agreement that ChAT is potentially the best marker to use for these purposes. Although successful purification and antibody development has proven to be a real struggle, it is apparent that there are now several highly specific antibody reagents against ChAT enzyme derived from a number of different species. The chapter presents information concerning the development of these reagents and discusses the results thus far on their usefulness in localizing cholinergic structures in mammalian brain. Of the monoclonal antibodies developed in the laboratory, one cross-reacts with enzyme derived from all mammalian species tested and has proven to be a very useful reagent for localization of ChAT immunoreactivity. This antibody has been employed to localize ChAT in rat, guinea pig, monkey, ferret, and mouse brain.
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